We have fabricated a MEMS device on suspended silicon nitride in order to measure the thermoelectric properties of single nanowire. The temperature gradient was generated by a nanoheater and the temperatures of thermometers were calibrated and measured. Seebeck coefficient and electrical conductivity of single Bi2Te3 nanowire with a 70 nm-diameter were measured in the temperature range of 50 K to 400 K. Based on the simulation results of the temperature distribution over the MEMS structure, the method of calibrating the temperature and measurement uncertainty are discussed.

We developed scanning electron microscopy with polarization analysis (SEMPA) system and utilized it to characterize the local magnetic probes used in the magnetic microscopy based on the scanning probe method. With a specially designed reference sample we could determine the Sherman function of spin detector and the uncertainty in measurement of spin polarization. Based on the measurement procedure established with the reference sample, we could characterize the local magnetic probes by measuring the magnetic domain images and the spin polarization of the magnetic probes.

We report on a high-sensitivity surface plasmon resonance (SPR) sensor based on phase measurements using rotating analyzer ellipsometry. In this work, the experimental setup for the SPR sensor was based on a custom-built rotating analyzer ellipsometer, which was equipped with a SPR cell and a microfluidic system. The most important feature of our system is that biomolecular detection sensitivity can be significantly enhanced by measuring the phase difference between the s- and ppolarizations. In this paper, we demonstrate how a custombuilt rotating analyzer ellipsometer in the SPR condition can be used to directly detect the interaction of D-biotin and protein tyrosine phosphatase (PTP) inhibitor with immobilized streptavidin and PTP maltose binding protein (MBP)-SHP2.

Atom lithography can be used to fabricate regular nanoscale pattern on a substrate with high resolution and large area. Because the pitch of the grating is traceable to absolute atomic frequency, it has potential to be used as nanoscale pitch standards. The researches on laser focusing chromium in national institute of metrology are reported in this paper. Chromium wire array with a pitch of λ/2 and a height of 1 nm has been deposited on InP substrate.

Cell counting is an important procedure in cell biology and its uncertainty evaluation is fundamental to improve cell-based measurement comparability which is currently variable. In this study, for the first time, the uncertainty of a metabolic assay for cell counting based on fluorescence intensity measurement has been evaluated. Results, in 2D cell culture, revealed that three are the main components: repeatability and reproducibility of the measurement system and a contribution due to the sample loading step procedure. This study represents the basis of a 3D cell culture in which metabolic methodologies are the most suitable method to monitor cell growth.