UNCERTAINTY ANALYSIS OF CELL COUNTING BY METABOLIC ASSAYS |
|---|
| C.Divieto, G.Sassi, M.P.Sassi |
- Abstract:
- Cell counting is an important procedure in cell biology and its uncertainty evaluation is fundamental to improve cell-based measurement comparability which is currently variable. In this study, for the first time, the uncertainty of a metabolic assay for cell counting based on fluorescence intensity measurement has been evaluated. Results, in 2D cell culture, revealed that three are the main components: repeatability and reproducibility of the measurement system and a contribution due to the sample loading step procedure. This study represents the basis of a 3D cell culture in which metabolic methodologies are the most suitable method to monitor cell growth.
- Keywords:
- 2D and 3D cell culture, scaffold, cell proliferation rate, CellTiter Blue, resazurin, uncertainty analysis
- Download:
- IMEKO-WC-2012-SS2-O11.pdf
- DOI:
- -
- Event details
- Event name:
- XX IMEKO World Congress
- Title:
Metrology for Green Growth
- Place:
- Busan, REPUBLIC of KOREA
- Time:
- 09 September 2012 - 12 September 2012